FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3590554
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15250.042.9637578
CTGTCGC18050.039.091259
GTCTTGC34500.033.804651
GTCTCGC16400.033.5690771
TCGCTCT18600.032.5150874
TCTCGCT20300.028.02312
CGCTCTG22100.027.365645
CTCGCTC20950.026.8047053
CCGTTCG1801.5945164E-423.99999
CTACGCT3352.2919266E-923.6410354
GTATTAG12600.023.5712681
GTGTTAG25300.023.47811
GTCTTAC15700.023.0695951
TTGCTCT48750.022.8915754
GTGTAGG19850.022.6256391
GTTAGCC21400.022.5403923
GCGTAGT3554.1745807E-922.4457441
GTTTCAC54550.022.0405373
GTATAAC7700.021.6374841
GTGTAAC11050.021.6332281
Produced by FastQC (version 0.11.5)