FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3590554
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13700.034.3261151
TCTGTCG14950.033.7079168
GTCTTGC32600.029.5269741
TCGCTCT16450.028.4460834
GTACTAT9350.028.2915131
GTGTTAC15700.028.0812761
GTATTAG11650.027.7518521
CTGTCGC18900.027.044049
TACATCG2405.653419E-826.9964285
GTATTAT11800.026.7763711
TCTCGCT19250.025.8043792
GTGTTAG25750.024.5406721
CTCGCTC19500.023.9968263
CGCTCTG19850.022.8483625
GTATAAT10400.022.6090281
GTGTAAC11400.022.5594461
GTCTCAC42400.022.3557431
GGGTTTC62300.022.2914851
GTACAAG12200.022.2847181
GTTTCAC57950.021.9884623
Produced by FastQC (version 0.11.5)