FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3734400
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16000.037.3532878
CTGTCGC18750.035.7151459
GTCTCGC16650.033.5048261
GTCTTGC37000.030.7417641
GTACCGA1804.961812E-628.002096
TCGCTCT18950.027.3582464
TCTCGCT20250.025.6032832
CGCTCTG19800.025.4564485
GTATTAA11750.024.6634031
TATACTG11250.024.3218175
CTCGCTC21700.023.8873483
GTCTCAC44150.023.4658451
GTTCTAC11750.023.4302331
GTGTAAC10850.023.3705641
TTCACCG9000.023.2017335
GTATTAC8600.022.7455371
GTGTAAA22150.022.5686841
GTGTTAC16100.022.499611
GTGTAAT15400.022.1109811
ACCGTGT10100.022.1009568
Produced by FastQC (version 0.11.5)