FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3734400
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC17100.035.2363971
TCTGTCG16200.035.10638
GTCTTGC36500.033.4186551
CTGTCGC18900.031.9956159
GTGTAAA23550.027.7698081
TCTCGCT20550.027.6750372
GTATTAA11300.026.661171
GTATAGA12500.026.4530831
TCGCTCT18400.025.8222034
TAATACT11800.025.6232624
GTATTAT10950.025.5001811
TTGCTCT52100.025.2860224
GTCTTAC15700.024.3375841
GTATAAC7100.023.8036331
CTCGCTC20900.023.766763
CTTGCTC51700.023.5322443
GATCGTT3702.8558134E-1023.348159
TTACACT18000.022.3966314
GTCTCAC45000.022.2075271
GTACTAT8350.022.0002331
Produced by FastQC (version 0.11.5)