FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3788778
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14750.042.4689378
CTGTCGC17950.038.1068739
GTCTTGC35400.034.9902571
GTCTCGC15800.032.5503431
CCGCGTA1352.247227E-532.000869
TCGCTCT17500.026.7425164
GTATTAG12750.026.7018871
GGTATAC9000.026.5598561
GTACTAT9250.025.8420261
GTATACG2851.000808E-825.416131
TCTCGCT19550.025.0448112
CTCGCTC19750.024.7879223
GTATAAT12250.024.2438771
GTACTAG7950.023.6897531
GTATTAT13050.023.3127271
GTCGTAC1600.002187462322.6362441
GTCTCAC45450.022.6312621
GTGTAGA20600.022.5043811
GTGTAAA24400.021.9682881
GTATAGG11450.021.5093731
Produced by FastQC (version 0.11.5)