FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3788778
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15150.041.3415158
GTCTCGC16250.038.9009631
CTGTCGC17550.034.0471539
TCGCTCT18500.033.0761954
GTCTTGC37600.030.887621
TCTCGCT20650.029.2838082
CTCGCTC21150.028.9318943
CGCTCTG23500.025.4260315
GTGTTAG26500.025.2412431
GTACTAT8550.024.9314251
GTATAGA10950.024.8372121
GTACGGA3601.5643309E-1024.5015741
GTGTTAC16250.023.9738481
GTATAAT11850.023.5711361
GTATTAC9050.023.5541
GTCTCAC45850.023.0854531
GTATTAG12750.023.0603071
TACACCG2852.979341E-722.7334845
GTATTAT12300.022.7087761
TTATACT11450.022.634214
Produced by FastQC (version 0.11.5)