FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3551936
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15300.035.7638788
GTCTCGC17450.031.4025331
TCGCTCT18900.030.856434
CTGTCGC18250.029.982879
GTCTTGC30750.026.730451
CTCGCTC20100.025.432253
TCTCGCT21850.025.3756282
GTCTCAC34600.024.798041
TCCGTCG1500.001556704923.9994498
GTCTTAC10600.022.4468081
TTGCTCT41700.022.100214
GAGTGCA88050.021.7507746
GGAGTGC82800.021.4777665
GTATAAG8800.021.3028241
CGCTCTG20300.021.2802965
GTCTACG3106.593091E-720.93281
GGCTGGA113300.020.6823541
TCTGTCA40000.020.339538
GTATAGT7100.020.3103851
GTATAGG7500.020.1885221
Produced by FastQC (version 0.11.5)