FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4496750
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG18050.040.2872548
GTCTCGC21100.038.559731
TCGCTCT22250.037.8598944
TCGCGAA800.002107973735.9992567
GTCTTGC39150.035.6786161
CTGTCGC21900.032.547279
CGCTCTG24550.031.9667535
CTCGCTC25700.029.1355843
GTATTAC10450.028.9381661
TCTCGCT26400.028.9078482
CGTTTCG2502.6411726E-928.7994069
GTGTTAG29350.027.2303281
GTCGCGA1400.001041333825.7137536
GTTTCAC66400.024.2886543
TAATACT13850.023.9128634
TTGCTCT56050.022.9932794
GTCTCAC55150.022.9776271
GTATTAA14200.022.817191
GTGTAGT24000.022.8002871
GTATAGT11400.022.737131
Produced by FastQC (version 0.11.5)