FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4496750
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG18750.045.310738
GTCTCGC20500.040.0972021
CTGTCGC24350.035.1858759
TCGCTCT22250.033.9765784
GTCTTGC40750.030.434341
GTATTAG13850.029.6748221
TCTCGCT25750.029.6442682
CGCTCTG23950.027.3562285
GTTTCAC66150.026.9924413
GGGTTTC72050.026.920391
GTCTCAC54450.026.4847071
GTATTAT12650.026.2198621
CTCGCTC27500.026.1810843
GTGTTAG30300.025.9386231
GGTATAC8750.024.7215821
GTATTAA12950.024.4988651
TTGCTCT56950.023.8939744
GGTTTCA72300.022.809162
GTATAAG12400.022.6780641
GTACTAG8000.022.532691
Produced by FastQC (version 0.11.5)