FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4879085
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG18600.048.3862538
GTCTCGC21950.043.624771
CTGTCGC22600.040.7780539
TCGCTCT24650.036.8026054
GTCTTGC46400.034.136641
TCTCGCT28650.032.165662
CTCGCTC27900.030.9672053
CGCTCTG27800.030.8196095
TTGCTCT64050.026.3039934
GTGTAAC13750.025.657211
CTTGCTC60850.024.4925923
GGTATAC9200.024.259951
GTGTTAG32350.024.2587411
GTCTCAC58000.023.9577141
GTCTTAC19050.023.0542451
GTGTTAC18750.023.0391271
GGGTTTC78600.022.8998971
TCTTGCT70300.022.018632
TCTGTCA60100.021.6834838
GTTTCAC71600.021.6197383
Produced by FastQC (version 0.11.5)