FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4879085
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG21100.045.041348
GTCTCGC21400.038.73771
CTGTCGC25650.037.8936279
GTCTTGC41650.034.9611851
TCGCTCT23350.033.917644
GTGTTAG31950.029.105071
GTACGGG4300.028.499041
TCTCGCT27800.028.4936332
CGCTCTG27600.027.1296425
CCGTGTT15600.026.768459
CGTTTCG2706.002665E-926.6658869
ACCGTGT16000.026.5492278
GTTAGCC25400.026.3614353
TTCACCG13750.026.1810535
CTCGCTC29550.025.8266583
GTATTAG14400.025.530391
TTGCTCT58800.025.34624
TACACCG3701.0913936E-1125.2965585
GGGTTTC71950.025.1473771
GTTTCAC68300.024.6669223
Produced by FastQC (version 0.11.5)