FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4851997
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC23050.041.9136731
TCTGTCG19850.041.34918
GTCTTGC41650.037.390441
CTGTCGC24650.037.3864979
TCGCTCT25850.034.8153844
TCTCGCT27950.031.4329242
CGCTCTG28950.030.0925185
GTCTTAC18700.027.759571
CTCGCTC29650.027.4394533
GTGTTAG32150.027.1347771
GTATTCG3006.2755134E-1026.435851
GTTAGCC26750.025.3003043
GGTATAC8650.025.0050071
GTGTTAC20050.024.452111
TTGCTCT58900.024.447614
GTATTAG13150.024.123971
GTGTATA22450.024.0861151
GGGTTTC73550.023.624151
GTCTCAC55400.022.9047071
GTGTAAC14000.022.65931
Produced by FastQC (version 0.11.5)