FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4335616
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17200.035.999128
GTCTCGC16900.034.5618441
GTCTTGC40600.033.5686871
CTGTCGC22550.029.6932899
TCGCTCT20600.027.9604844
TCTCGCT22400.026.0392842
GTACTAC7600.025.6182081
GTATAAG11900.025.4507681
GTGTTAT14800.023.8744241
CGCTCTG23100.023.6877335
GTGTTAC16800.023.6076071
GTCTCAC51650.023.5947271
GGAGTGC105200.023.543155
GTATAGG11100.023.3871941
TTGCTCT54900.023.212554
GAGTGCA114950.023.0494586
GTGTTAG27300.022.9802721
CTATCCG2552.8790346E-622.5876859
GTATAAC7150.022.1878471
CTCGCTC23850.022.0371973
Produced by FastQC (version 0.11.5)