FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4363308
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15800.042.379858
GTCTCGC20000.039.5987321
GTCTTGC39850.035.592341
CTGTCGC20850.035.2231069
TCGCTCT22700.034.255594
GTATTAA12950.030.578171
TCTCGCT24350.029.567842
CTCGCTC24650.028.9169043
CGCTCTG25250.028.229775
TTGCTCT54600.025.450614
GTGTAAC13450.025.1590441
GTGTTAG30000.024.4792161
GGGTTTC71400.024.4025821
GTATAGA13050.024.2750851
GTATAAT10150.024.1174561
GTCTCAC52150.023.7461241
GTGTTAC17650.023.65931
GTTTCAC62750.023.2924843
TCGATCT18000.023.2000569
GTACTAG9300.022.4508951
Produced by FastQC (version 0.11.5)