FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4363308
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC19550.042.7853971
TCTGTCG17900.039.8202638
TCGCTCT22500.036.7991074
GTCTTGC40300.034.891041
CGCTCTG24350.032.22925
CTGTCGC23000.031.929669
CTCGCTC24550.031.6733673
GTATTAG13500.031.5139331
TCTCGCT24850.031.2952942
GTATTAT13550.027.6724991
GTCTCAC51700.025.5237771
GTGTAAC14850.024.7644161
TTCACCG13700.024.1745975
GTGTAAA26700.024.036051
GTGTTAC16800.023.6068341
TTGCTCT54350.023.4474534
GTATAAA17000.023.329111
GTATAGT11150.023.2815571
ACCGTGT15550.023.1505628
GGGTTTC70450.023.1319271
Produced by FastQC (version 0.11.5)