FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4158187
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG18350.047.8677448
CTGTCGC20900.044.438839
GTCTCGC20200.040.6324231
TCGCTCT22100.036.487574
GTCTTGC38200.036.375851
CGCTCTG23900.031.3295865
TCTCGCT25100.030.6919212
CTCGCTC27000.027.9991443
GTCTTAC16800.026.5706161
GGTATAC7900.026.4295731
GTGTTAC17450.026.4060691
GGGTACG3006.384653E-1026.3991931
TTGCTCT52550.026.0315534
GTATAGC8900.025.886851
GGGTTTC66450.024.920231
TTACACT20150.024.2970244
GTGTTAG26900.024.0884821
CTTGCTC51600.023.9992683
GTTTCAC59650.023.8986823
TCTGTCA53650.023.8874368
Produced by FastQC (version 0.11.5)