FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4158187
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG18750.054.910598
GTCTCGC19800.047.709721
TCGCTCT20400.045.8811764
CTGTCGC25050.040.2384879
TCTCGCT24150.038.1646422
CTCGCTC24300.036.443513
GTCTTGC40200.034.6203961
CGCTCTG25050.034.2027175
GTTTCAC59150.028.7262293
TTCACCG11450.027.6674125
GTATTAA11100.027.2851961
TTGCTCT53700.026.5468044
GGGTTTC65850.025.5153161
GTCTCAC49700.025.3911531
GTATTAG14050.025.1489891
ACCATGT51900.024.9704578
CTTGCTC53450.024.5157413
GTATAGA10950.024.3662321
TCTGTCA52500.023.9993848
GTCTTAC16100.023.7383631
Produced by FastQC (version 0.11.5)