FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3977027
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC18200.047.5366821
TCTGTCG15950.043.3345268
TCGCTCT19650.041.4037174
CTGTCGC19250.037.401829
TCTCGCT23550.036.0811272
CTCGCTC23550.034.2413253
CGCTCTG22350.033.82485
GTCTTGC37300.033.4392361
GTACCGA1601.9624495E-631.542571
GTATTAA10500.027.4656411
GGGTTTC63900.026.2890011
GTTTCAC57150.026.0781973
GTGTTAC17500.025.9550271
GTTAGCC22600.025.1676183
GTATTAC9250.024.941771
CGTTTCG1751.3172785E-424.6852029
GTATTAG10900.024.4733961
GTCTCAC46100.024.0845641
GGTATAC8450.023.8902321
TCTGTTG43600.023.7793248
Produced by FastQC (version 0.11.5)