FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3632718
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14850.050.4232948
CTGTCGC18550.044.6352779
GTCTTGC33350.036.2691841
GTCTCGC16250.034.5593451
TCGCTCT18250.028.4049474
GTTTCAC50550.027.7739583
TTGCTCT45450.026.9301854
GGGTTTC58650.026.8844051
GTATTAA8600.026.7901941
GTGTTAG24100.025.3937131
GTATTAT9800.024.979121
GTCTCAC43600.024.7701781
GTGTAAT14300.024.6708621
TCTCGCT19200.023.9992182
CTTGCTC46450.023.8703783
GTTAGCC19100.023.7482453
GTATTCG3052.194247E-823.6061131
CTCGCTC22050.023.1832373
TCTTGCT52100.022.939752
TCGATCT13850.022.8732159
Produced by FastQC (version 0.11.5)