FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3080557
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13650.037.4488648
CTGTCGC18500.027.631199
GTCTCGC14100.027.6301821
GTCTTGC26600.027.122361
GTCTAGC11050.026.768911
TCGCTCT14600.026.6298034
GTATAAG6300.026.3388231
TTGCTCT35150.023.7607294
GTGTTAG17700.023.640891
GTTCCGA2151.9395506E-523.4892251
TCTCGCT16050.022.9183942
GTGTTAC10350.022.305851
GTTACAC8100.022.2544543
CTCGCTC16550.022.219493
GTCTTAC10100.022.1436611
TCGAACT20550.021.7216519
GTCTAGA12750.020.9363351
CGCTCTG17800.020.6289565
CTCGAAC21300.020.6187928
CGTCAGA4551.6370905E-1020.6129951
Produced by FastQC (version 0.11.5)