FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3469375
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13900.041.4372948
GTCTCGC16400.037.3835561
TCGCTCT17250.035.0595554
GTCTTGC31800.029.7132231
TCTCGCT19700.029.646532
CTGTCGC18900.029.3322339
CGCTCTG20350.027.2422225
GTGTTAC16300.026.992791
CTCGCTC21600.026.6941343
GTGTAAT14500.025.8666921
GTCTTAC13250.025.040761
GTGTTAG22700.024.7841641
TTATACT8800.024.5445334
GTGTAAC10650.024.381391
GTTTCAC49600.024.1215933
GGTATAC7250.023.8769451
GTATTAG10600.023.8159391
GTCTCAC40900.023.8076211
GTGTAAA18900.023.6611291
GGGTTTC57300.023.1616121
Produced by FastQC (version 0.11.5)