FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3469375
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14800.042.3841821
TCGCTCT16850.038.4558564
TCTGTCG15500.038.0903178
TCTCGCT19400.032.3338552
CGCTCTG20550.030.4808675
CTCGCTC19900.030.0292783
GTCTTGC32300.029.0193081
TACTGCG3500.028.7999947
CTGTCGC21300.028.0563349
GCGTAAC1555.7147307E-527.9103831
GTATTAG11400.027.8287731
GTGTAAT13150.026.8668381
GTCTTAC14400.025.5360621
GTATAAT9950.024.637811
GTGTTAG21850.024.4189241
GTATTAC8050.024.1832221
GGGTTTC52250.023.7349551
TTGCTCT43450.023.6955154
GTACGGC2452.0082607E-623.5434511
GTCTCAC42400.023.4671021
Produced by FastQC (version 0.11.5)