FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2394311
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT37380.1561200696150166No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10000.036.718768
GTCTCGC11400.035.43161
TCGCTCT13350.031.2811494
CTCGCTC13150.029.0507933
CTGTCGC12700.028.3454999
TCTCGCT14100.028.1258582
GTCTTGC20750.025.7229771
GTCTCAC24950.025.4401511
CGCTCTG13500.024.5335275
GTCTTAG8900.024.3130151
GTCTAAA7850.022.9708961
GTATTAT5150.022.408891
GTCTTAC8400.022.3255211
TCACTCT31050.021.565394
GTATAAA6750.021.371441
GTCGAAA1700.003187576821.2142981
GTATAAT4101.0022632E-921.1108111
GCGTAGT3809.61154E-920.8793351
ATAACCG1750.003809750320.5715925
GTCTAAG7550.020.0622621
Produced by FastQC (version 0.11.5)