FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2394311
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT10500.030.1706984
GTATAAG5250.028.8414651
GTCTCGC10300.028.0014231
GTCTTGC22100.026.4271321
TATTCCG1801.5945162E-423.999425
TTGCTCT27850.023.7839834
CGCTCTG12200.023.6059865
GTCTCAC23350.023.468431
GTGTTAG12750.023.1862741
CTCGCTC12600.022.8565883
TCTGTCG10150.022.698968
GTATTAA5800.022.3769991
TCTCGCT13250.022.3108522
GTATTAG5900.021.9977261
GTCTATA4058.6401997E-1021.3640461
GTGTAAT6750.021.3640461
GGAGTGC53950.020.8187735
TAATACT4501.4551915E-1020.7994964
GTGTAAG10500.020.6010461
GAGTGCA59050.020.1180556
Produced by FastQC (version 0.11.5)