FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2906470
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11650.043.8782048
GTCTCGC11950.037.427021
GTCTTGC28400.032.766711
CTGTCGC15700.031.642149
TCGCTCT13600.030.7052734
GTGTTAC10900.027.7961251
CGTTTCG1306.7552045E-427.6912829
TCTCGCT14650.026.5807932
GTGTAAT12150.024.9364431
TTGCTCT36650.024.163234
GTATTAC6300.024.0458561
GTCCGGT2701.7330422E-724.0458551
TCACCGT11250.023.0395436
GTATAAC5950.023.0355261
CGCTCTG15450.022.8344975
CTCGCTC16450.022.788833
TAAACCG1902.3001536E-422.736395
GGGTTTC49500.022.7342621
GTTTCAC44100.022.7227463
GTGTTAA11450.022.6808071
Produced by FastQC (version 0.11.5)