FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2906470
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12450.038.1682058
GTCTCGC13350.037.2713741
GTCTTGC25100.030.4536761
GTATAGG6750.029.9131281
CTGTCGC16250.029.6857919
TCGCTCT15100.029.0852364
GTGTAAC7750.028.84481
ACCGTGT9750.028.0611938
TCTCGCT16700.027.2039322
GTTAGCC15400.026.6485633
GTGTTAG17950.026.5147231
TAACACT14050.025.6220194
GTATACG1701.0702766E-425.4512961
GTGTTAC11950.025.3448051
GTACTAG5200.024.9618471
GGGTACG2651.4515899E-724.490871
CTCGCTC17450.023.9305253
TGTTAGC18350.023.5788572
CGCTCTG16400.023.2676075
GTATTAC5900.023.222511
Produced by FastQC (version 0.11.5)