FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2407799
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT45660.18963376926396266No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9400.033.7008178
GTCTCGC10350.032.756281
TCGCTCT12700.030.0460744
GTATAAG7500.029.8151861
CGTCAGA3851.8189894E-1124.356771
CTGTCGC13400.024.1781649
GTCTTGC19750.023.7401431
GTACTAG4600.023.5217971
TCTCGCT13350.023.22582
CTCGCTC14050.023.089813
GTCTCAC26750.022.9209291
GTGTAAC7250.022.8837341
GTCTTAC10150.022.74161
CGCTCTG13350.022.6508055
GTTTAGA8800.022.1318721
GTGTTAG15450.021.9435271
GTATTAT6650.021.6942881
GTATAGG5750.021.3264271
GTACGGG2404.540047E-521.038941
TACGGGA2455.336007E-520.6022872
Produced by FastQC (version 0.11.5)