FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2407799
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC21000.027.1227651
CGTTTCG2207.958697E-726.1822419
GTCTCGC9800.025.013761
TCTGTCG9900.024.727678
GTATTGG7950.023.5793781
TCGCTCT12600.022.8565624
GTGTAAC6950.022.8225441
GGGTTTC36150.022.1381231
GTACTAT4307.8216544E-1121.7972151
GGTATAC4307.8216544E-1121.7972151
CTCGCTC12250.021.7463873
GTATAAG6650.021.6837541
GTATTAA6000.021.6295471
TTGCTCT30200.021.456414
GTTTCAC34100.020.9026953
TCTCGCT12100.020.8549332
CGCTCTG12050.020.3148385
GTCTACG2506.239976E-520.1875741
CCGCGGA2155.29999E-420.0933469
GTGTTAG14550.019.820891
Produced by FastQC (version 0.11.5)