FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2762062
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11850.042.5324638
GTCTCGC11850.038.9573481
TCGCTCT14000.036.5130044
CTGTCGC15000.033.6006479
GTTTCAC39450.028.2879833
TCTCGCT15450.027.5435452
GTCTTGC28000.027.0494881
GGGTTTC42450.027.0176281
CGCTCTG16950.026.7601245
CTCGCTC16400.025.901533
GTATTAT9300.025.5952171
GTATAAT7600.023.7276211
GTGTTAG19200.023.66831
GTATTAA8550.023.6221661
GTCTCAC32800.023.5308551
GTGTTAC12300.023.4575521
TTCACCG7700.023.3758035
GCGTTAC1550.001863655423.2683751
GGTTTCA41750.023.149662
ACCGTGT9050.023.0722688
Produced by FastQC (version 0.11.5)