FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2702678
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT29290.10837399053827351No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11600.050.2742928
GTCTCGC12900.037.4572451
CTGTCGC15350.037.054229
GTCTTGC23200.031.7075651
TCGCTCT14500.031.2829424
GTATTAA6100.029.5570351
TCTCGCT16050.028.7482092
CTCGCTC16200.026.6964573
TTGCTCT33800.025.9883164
CGCTCTG16750.025.7911935
GTCTCAC28850.022.4981821
GTCTAGC9950.022.4692881
GTGTATA9650.022.4204671
CTTGCTC32650.022.2974683
GTCTTAC9750.022.1905141
GTATTAT5200.022.1905121
GGCTAGA16150.021.8813571
GTGTTAG16050.021.568351
GGCGAAT2353.8660713E-521.4823061
GTATTAG5800.021.1383761
Produced by FastQC (version 0.11.5)