FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2702678
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13500.037.393241
GTCTTGC22850.035.663261
TCTGTCG11500.031.9301978
TCTCGCT16200.028.0438922
TCGCTCT14650.028.0129244
CTGTCGC17050.025.3370559
CTCGCTC16000.024.7493553
GTATTGA5650.024.2512421
GAGTACG1902.2749549E-422.7733291
GTGTTAC8550.022.7733271
GTGTTAA8550.022.7733271
GTATAGT6050.022.6478541
CGCTCTG15600.022.6147965
GTACAAA10300.022.4048271
GTCTCAC27750.022.3493211
TTGCTCT31650.021.8382954
GAGTGCA64900.021.4112436
GTATTGC5750.021.3211151
GGAGTGC62500.021.196255
GTACGGA2053.8027889E-421.1069871
Produced by FastQC (version 0.11.5)