FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3560284
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16050.046.6526688
GTCTCGC14500.039.7865261
CTGTCGC20900.037.5490049
GTCTTGC32850.037.3187941
TCGCTCT15950.032.5009544
GTATTAG12100.030.3947681
TCTCGCT18750.029.6044432
GTCTCAC44550.028.1653791
CTCGCTC18450.028.1246783
GTATAGA10050.027.2666361
GGGTTTC60250.026.0923671
GTACTAA7250.025.8612421
GTTTCAC53850.025.829923
GTGTTAG24550.025.2616081
GTCTTAC14600.024.6962591
GTATTAT10850.024.5915571
TTGCTCT48400.024.2474754
CTCGATC13800.023.9992088
GTGTAAG17850.023.8356951
GTATAAC6700.023.6789231
Produced by FastQC (version 0.11.5)