FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3560284
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16800.050.142768
GTCTCGC15200.041.2788541
CTGTCGC23100.037.714219
GTCTTGC31850.035.5502131
TCGCTCT17250.032.5555464
GGTATAC7600.030.3660531
CGCTCTG19900.028.2202595
CTCGCTC19600.027.5501923
TTGCTCT42800.026.915084
TCTCGCT20100.026.1918752
GTATTAT12750.026.019541
GTATAAT10900.025.804181
GTCTCAC40950.025.3606621
GTATTAA8950.024.9798971
GTTTCAC51600.024.9759943
GTGTTAC16150.024.5607781
GGGTTTC57400.024.5004841
GTATTAC8100.024.0397931
GGGTTAT10550.023.9258611
TATACTG10050.023.641085
Produced by FastQC (version 0.11.5)