FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3288578
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC29750.040.723991
TCTGTCG14650.036.8596428
GTCTCGC15200.036.5321431
TCGCTCT17050.033.782094
CTGTCGC17350.031.123569
TCTCGCT19000.028.4653032
CGCTCTG18650.027.7955045
TTGCTCT42200.026.7860154
GTGTTAG24550.026.7311671
GTATAAG9250.025.7276551
CTCGCTC20500.025.638343
GTTTCAC47700.025.2068753
GTATTAG11450.025.1931511
CTTGCTC41700.024.8626443
GTGTTAC14800.024.8505781
GGGTTTC52600.024.404071
GTATAGT9050.023.9056571
GTCTTAC13800.022.9933171
GTGTAAC11300.022.9748171
TCTTGCT47150.022.9412842
Produced by FastQC (version 0.11.5)