FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3142298
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13400.043.5230758
GTCTCGC15150.038.0276641
CTGTCGC15700.035.312669
TCGCTCT16950.035.2555124
GTCTTGC28200.034.219841
CGCTCTG18400.032.8685155
TCTCGCT18000.031.9989782
GTATTAG11250.029.4460891
GTATCGC2253.011337E-828.8059561
CTCGCTC19550.027.6206023
GTGTTAT11600.026.0743581
GCGTAAC1751.3151595E-424.6908191
TACTGCG3851.8189894E-1124.3120757
TTGCTCT41950.024.1994654
GTCTTAC12850.024.0983681
GTCTCAC34750.023.6250291
GTTTCAC43000.023.6085473
GTGTTAG20400.022.9459191
GGGTTTC51500.022.7930621
GTATTAT8600.022.6093251
Produced by FastQC (version 0.11.5)