FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3142298
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15450.047.999858
CTGTCGC18900.041.9046339
GTCTTGC30900.038.2134741
GTCTCGC15100.037.668761
TCGCTCT16400.032.4877054
GTTTCAC43850.029.2268183
GGGTTTC51550.027.9339581
TCTCGCT18700.027.721842
TTGCTCT41150.027.1202074
GTCTTAC11350.026.643091
CTCGCTC19150.026.3184573
GTACTAC5500.026.1817361
CGCTCTG19350.026.046435
GGTATAC4900.024.9795151
GTGTTAG20700.024.347751
GTGTAAA17450.024.3437611
TTCACCG8300.024.2890825
GGTTTCA49150.023.7313612
CTTGCTC42500.023.2093413
ACCATGT40100.022.9824738
Produced by FastQC (version 0.11.5)