FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3478579
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13950.047.485988
GTCTCGC15900.044.8437841
CTGTCGC17050.038.8521659
TCGCTCT15900.037.1310584
TCTCGCT18250.035.9003832
GTCTTGC33150.034.109891
CTCGCTC18000.033.199093
CGTTTCG2151.9386789E-830.1408759
CGCTCTG19050.029.1015625
TTGCTCT46000.027.2340354
GGGTTTC56050.026.9840971
GTATAAC7600.026.534361
GTTTCAC53850.026.3391113
TTCACCG11400.025.8940265
GTCTCAC39750.025.5473671
CTTGCTC42100.025.4814853
CTCGATC14500.025.3252628
GTATTAT11100.025.304971
TCGATCT14500.024.828699
GTATTAC9300.024.7817081
Produced by FastQC (version 0.11.5)