FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3478579
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC16000.044.549611
TCTGTCG14450.041.3566328
TCGCTCT16600.039.9038354
GTCTTGC31050.037.3330081
CTGTCGC17850.033.8825459
CTCGCTC19350.032.3722723
TCTCGCT20500.032.311912
TATCGCG900.003754451232.000185
CGCTCTG18950.031.9157475
GTGTTAC14650.027.5219441
TTCACCG10200.026.8236795
GGTATAC6500.026.5843811
GTCTTAC12650.026.1815871
GTTTCAC52300.026.1569353
GTGTTAG21200.026.1507151
TTGCTCT44600.025.829744
GGGTTTC55050.025.6346511
GTGTAAC11550.024.9348471
CTGTTGC36650.024.5567539
CTTGCTC43700.024.2198143
Produced by FastQC (version 0.11.5)