FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3350923
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12850.050.4291238
CTGTCGC16400.040.3911329
GTCTCGC14350.037.1347241
TCGCTCT16000.035.0997244
GTCTTGC31750.032.4334141
TCTCGCT16700.031.0416722
CTCGCTC18800.027.1912753
TACACTG21500.027.1253685
GTATTAT9550.026.3915581
GGGTTTC52800.026.3222961
GTTTCAC48650.026.1950663
CGCTCTG18700.026.181615
GTATAGA9400.026.0466211
GTGTTAC14900.025.1314471
GTCTCAC36350.024.961261
GTACTAG7050.024.514471
TTGCTCT42700.024.280844
TTACACT16900.023.8578014
CTACACT15800.023.6960184
GGGTACG3352.2864697E-923.6454871
Produced by FastQC (version 0.11.5)