FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3350923
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13350.041.528091
TCTGTCG13500.038.9333348
GTCTTGC29950.035.5793041
TCGCTCT16400.033.804884
GTATTAG10500.033.61
CTGTCGC17850.031.4621839
CGCTCTG18350.030.2125345
CTCGCTC17850.028.6386553
TCTCGCT19150.027.8224532
GTGTTAG20400.026.4705891
GTCTTAC14600.025.1506821
GTCTCAC37700.024.6366041
GTGTAAT12400.023.8064521
GGGTTTC53300.023.504691
GTGTTAC14100.023.489361
CGAATAC2802.5080408E-723.1428593
GTTTCAC48900.022.9693243
GTACTAA7550.022.8874171
AATACCG3153.088644E-822.8571435
GTATTAT9650.022.3834191
Produced by FastQC (version 0.11.5)