FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2924688
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11650.044.497788
CTGTCGC13950.039.2257429
TCGCTCT14500.036.248224
GTCTCGC13850.034.3104131
GTCTTGC27550.034.2358781
GTGTTAG18100.032.220941
CGCTCTG16650.029.837795
CTCGCTC17500.029.211383
TCTCGCT16900.028.9703662
CGTTTCG1607.162197E-526.9999569
GTATTAG8900.026.6965851
GTATAAC5950.026.6218051
GTCTCAC35550.025.3164141
GTGTTAC12600.025.1428171
GTGTAAA17400.024.4137521
TAATACT9450.024.380914
GTCCGGA3551.6916601E-1024.3379881
GTTTCAC44250.024.2440283
TTGCTCT38500.024.1246344
GTTAGCC15800.023.6961613
Produced by FastQC (version 0.11.5)