FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3071956
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12250.048.197768
GTCTCGC12950.043.377631
CTGTCGC15200.041.2121059
TCGCTCT15100.040.0525554
TCTCGCT16450.031.9510272
CTCGCTC17250.030.4692423
GGTATAC6200.030.2010941
GTCTTGC29900.030.1078551
GTATAAT11200.027.0067461
CGCTCTG18800.026.8082265
GTACTAT7400.024.3304021
GTATAAC6550.024.1892491
GTCCTAC9850.024.1278551
ATAATAC10050.023.6415393
GTATAGA8700.023.1782051
GTATTAC6900.022.9622571
GTATTAG11000.022.9148161
GTCTTAC12950.022.8010621
GTATAGG8100.022.2277761
GTGTTAC12700.022.115761
Produced by FastQC (version 0.11.5)