FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3029531
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13400.038.697431
TCTGTCG14100.036.7659658
TCGCTCT14650.035.8777284
CTGTCGC17300.032.0462469
CGCTCTG16250.030.5728155
TCTCGCT17000.030.4946252
GTCTTGC28900.030.4029981
CTCGCTC16800.028.2861843
TAATACT8450.026.414644
GTATTAG12200.025.9745041
GTGTAAA18750.025.3511181
TTGCTCT39200.025.1636854
GTGTAAT13250.025.0032441
GTAATAC7050.024.5110473
GTATAGA10000.024.4868741
GTATTGG9850.024.1286011
GTCTTAC11800.023.192951
GTGTAGA17200.023.0297221
GTATAAT7850.022.9363731
GTACTAG5200.022.1600671
Produced by FastQC (version 0.11.5)