FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3029531
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14500.044.1929638
CTGTCGC16350.039.63299
GTCTCGC13750.035.0835271
TCGCTCT14900.034.7918364
GTCTTGC30250.033.5602231
GTATAAT8200.030.7316061
CTCGCTC16400.030.2925833
GTCTTAC13750.027.752641
TCTCGCT16950.027.6105332
CGCTCTG17850.027.4284845
TTGCTCT37000.026.6593764
GGTATAC6350.026.0786571
GTGTACG3607.2759576E-1225.999921
GTATTAT8700.025.655091
GTCTCAC35050.024.6504211
GAGTGCA77450.023.6125776
GGAGTGC70750.023.609825
GTGTTAA12850.023.5329971
GTGTTAC11050.023.4569381
GTACTAG6200.023.225731
Produced by FastQC (version 0.11.5)