FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2806660
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12450.045.6998631
TCTGTCG11900.044.1681568
TCGCTCT14500.041.7096824
GTCTTGC25800.036.2894861
CTGTCGC14900.035.2752389
TCTCGCT16450.035.0146452
CTCGCTC16600.033.397063
GTATAAT8000.032.40931
CGCTCTG16300.031.3614655
TTGCTCT32750.029.6789194
GTTTCAC38550.028.3886573
GGGTTTC45400.027.6026361
GTATTAG8300.026.8992841
GTGCGTA2156.4969026E-726.7983881
TTCACCG8600.026.7902745
CTTGCTC32900.026.6986663
GTATAGG6550.026.3892541
GTGTTAC9850.026.3222751
GTATTAA7900.025.5263121
ACCGTGT9500.025.0105768
Produced by FastQC (version 0.11.5)