FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2806660
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12350.043.1413271
GTCTTGC24700.039.6433831
TCTGTCG12900.038.5112958
TCGCTCT15100.037.191734
CTGTCGC16000.033.2997139
CTCGCTC15500.032.515853
TCTCGCT16500.031.8542692
CGCTCTG16750.030.519145
TTGCTCT33450.029.9190244
GTCTTAC9900.029.8179231
ACCGTGT9550.029.402898
GTCTAAT7350.028.4079171
TTCACCG8200.028.0973195
GTGTTAG18150.027.7683561
CCGTGTT9700.027.463689
CTGTTGC29100.026.721429
GGGTTTC42750.026.2734571
GTCTCAC32650.026.2417341
GTACTAT7150.026.1815931
GTATAGA7750.026.012681
Produced by FastQC (version 0.11.5)