FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2928580
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11650.050.0598378
CTGTCGC16000.040.9497959
GTCTTGC27650.040.1010671
GTCTCGC14650.038.8257451
TCGCTCT15200.035.526144
TCTCGCT16750.031.378952
CTCGCTC17500.031.2684173
CGCTCTG17100.030.3156385
GTCTTAC12650.030.1658571
GTGTTAC13050.027.0343481
TTGCTCT42250.026.9253084
GGGTTTC46100.025.7699381
GTCTCAC35600.025.4830191
GTATTAA8600.025.1161541
GTTTCAC43450.025.021743
TCTGTCA40650.024.6198028
CTTGCTC38550.024.4668053
TCTTGCT42700.024.2809092
GTGTTAA12500.024.191881
GTATTAG9050.023.8672851
Produced by FastQC (version 0.11.5)