FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2756785
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13050.045.8091051
TCGCTCT14000.042.685144
TCTGTCG10500.041.1437958
CGTTTCG1105.5231903E-639.2736249
CTGTCGC12400.038.9041149
TCTCGCT15350.037.0548742
CTCGCTC16250.035.0026053
CGCTCTG15350.034.709635
GTCTTGC24800.031.6562211
GTCTTAC10050.030.8167341
GTACTAT6100.030.699251
GGGTTTC48100.028.0014651
GTTTCAC43150.026.530353
GTGTATA13350.025.8966851
GTCTCAC32050.025.1694761
ACCGTGT8150.024.736768
GTGTAGA13400.024.7250561
TTCACCG8000.024.2996715
TTGCTCT34650.023.6879924
GGTTTCA46650.023.3051532
Produced by FastQC (version 0.11.5)