FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2756785
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12250.045.2571458
GTCTCGC10800.036.6666681
CTGTCGC15400.036.4675339
GTCTTGC25150.035.2127231
TCGCTCT13250.034.777364
CGCTCTG13050.029.7931065
GTATAAT7250.028.8000011
GTTTCAC41200.028.6601943
GTTACGC1306.753652E-427.6923083
GTACTAT6550.027.4809171
GGGTTTC44300.027.304741
CTCGCTC14350.027.0940763
TTGCTCT34050.026.4317174
TAATACT7700.026.1818184
CTTGCTC34550.026.0492043
CGTTTCG1959.24523E-625.8461539
TCTCGCT14650.025.5563132
GTCTTAC11350.024.7400881
GTATAGA8100.024.01
GGTTTCA43550.023.641792
Produced by FastQC (version 0.11.5)