FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2530742
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11850.043.135848
GTATTAC4750.039.409761
GTCTCGC11750.037.9907531
CTGTCGC14350.037.1259969
TCGCTCT11550.034.906344
GTCTTGC22600.034.4064061
TCTCGCT13200.031.6357462
GTGTTAG17650.031.0022281
CTCGCTC12850.030.256223
GTTAGCC13550.028.6931673
CGCTCTG13300.028.6894685
GTATTAA7800.025.8456521
GTATTAG8850.025.2198491
TGTTAGC16000.025.1995092
GTCTCAC28900.024.913011
GTATAAA10750.024.7809121
GTATAGA8000.024.2995261
GTATATA11900.024.201211
GTGTAAA15500.023.689861
GTGTATA13000.023.2610841
Produced by FastQC (version 0.11.5)